Carboxyhaemoglobin: a possible reference material for haemoglobin assay.
نویسندگان
چکیده
The inter- and intralaboratory quality control of haemoglobin estimation in remote laboratories requires a more rugged control haemolysate than is commercially available. The stabilities of oxyhaemoglobin and carboxyhaemoglobin forms of an ethanediol-containing haemolysate were studied over a three-year period. From the results obtained, carboxyhaemoglobin under nitrogen is proposed as a possible candidate reference material for haemoglobin assay.
منابع مشابه
Single breath transfer factor for carbon monoxide in an asymptomatic population of never smokers.
BACKGROUND Data on reference values of transfer factor variables in general populations of asymptomatic never smokers are limited. The aim of this study was to examine the relation between test variables and age, height, haemoglobin concentration and carboxyhaemoglobin concentration. METHODS Measurements of single breath transfer factor for carbon monoxide (TLCO) were obtained for a randomly ...
متن کاملDetermination of the carboxyhaemoglobin saturation of blood by spectrophotometric analysis.
The spectrophotometric method of Commins and Lawther (I965) for the determination of carboxy-haemoglobin saturation in blood was found to lead to systematic undervaluation, because of dissociation of the carboxyhaemoglobin, and physical dissolution of the carbon monoxide in the solvent used for the analysis. The theoretical relationship between the true and apparent carboxyhaemoglobin saturatio...
متن کاملA colorimetric method for the determination of carboxyhaemoglobin over a wide range of concentrations.
A colorimetric technique for the determination of carboxyhaemoglobin in blood is described. Carbon monoxide released from blood in a standard Conway unit reacts with palladous chloride/arsenomolybdate solution to produce a blue colour. Using 0.5 to 2 ml. of blood, the method will estimate carboxyhaemoglobin accurately at levels from 0.1% to 100% of total haemoglobin and in the presence of other...
متن کاملDistribution of carboxyhaemoglobin concentrations in smokers and non-smokers.
Carboxyhaemoglobin concentrations were measured in 3487 subjects comprising 1255 non-smokers, 1933 cigarette smokers, 193 cigar smokers (39 primary, 154 secondary), and 106 pipe smokers (30 primary, 76 secondary). In cigarette smokers the mean carboxyhaemoglobin concentration was 4.78% of the total haemoglobin and 94.7% of smokers had a concentration greater than 1.7%. Primary cigar smokers had...
متن کاملA new method of carboxyhaemoglobin determination.
A quick and accurate method of determining the concentration of carboxyhaemoglobin (COHb) in blood has been developed. The method uses a dual wavelength double beam spectrophotometer in the 1st derivative mode, linked to a digital voltmeter (DVM), with the two beams set 3 nm apart around an isobestic point of reduced haemoglobin (Hbred) and carboxyhaemoglobin at 579 nm. The 1st derivative mode ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of clinical pathology
دوره 35 3 شماره
صفحات -
تاریخ انتشار 1982